Composite

Part:BBa_K2928007:Design

Designed by: Lauren Hartz   Group: iGEM19_ASIJ_Tokyo   (2019-10-15)


0.19 Wheat Albumin with expression using TDH3, RBS, Secretion Tag, and ADH3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to pick promoters and terminators of appropriate strength. Additionally, we made the decision to include a secretion tag in order to observe how the protein would be synthesized and act in a system in comparison to a construct without a secretion tag.


Source

The majority of the basic parts comprising this construct are previously existing parts on the registry. The sequence for the 0.19 WA was found on the Protein Database.

References